Journal: Journal of Cellular and Molecular Medicine

Article Title: Silencing of Cholesterol 25‐Hydroxylase Attenuates Lipopolysaccharide‐Induced Cardiomyocyte Damage In Vitro

doi: 10.1111/jcmm.70959

Figure Lengend Snippet: Cholesterol 25‐hydroxylase (CH25H) overexpression exacerbated oxidative stress, and CH25H knockdown attenuated the effects of lipopolysaccharide (LPS) on oxidative stress. (A–D) Effect of CH25H on oxidative stress markers (catalase [CAT], malondialdehyde [MDA], and reactive oxygen species [ROS]). Panel D shows representative flow cytometry images for ROS analysis. * p < 0.05, n = 3.

Article Snippet: Malondialdehyde (MDA) content was determined using an MDA ELISA kit (E‐EL‐0060, Elabscience).

Techniques: Over Expression, Knockdown, Flow Cytometry

Journal: Journal of Cellular and Molecular Medicine

Article Title: Silencing of Cholesterol 25‐Hydroxylase Attenuates Lipopolysaccharide‐Induced Cardiomyocyte Damage In Vitro

doi: 10.1111/jcmm.70959

Figure Lengend Snippet: CH25H overexpression exacerbated lipopolysaccharide (LPS)‐induced oxidative stress, mitochondrial dysfunction, and apoptosis, and MCC950 counteracted the effects of CH25H overexpression. (A) Representative images of Western blot for cleaved caspase‐1 and pro‐caspase‐1. (B and C) The statistical result of relative protein expression levels relative to GAPDH. (D) The statistical result of cleaved caspase‐1/pro‐caspase‐1 ratio. (E) Catalase (CAT) activity. (F) Malondialdehyde (MDA) level. (G) ATP level. (H) Mitochondrial complex I activity. (I) The bar graph of the statistical results of the apoptotic cell rate. (J) The representative picture (scale bar = 100 μm) of Hoechst 33258 staining. * p < 0.05, and ns p > 0.05, n = 3.

Article Snippet: Malondialdehyde (MDA) content was determined using an MDA ELISA kit (E‐EL‐0060, Elabscience).

Techniques: Over Expression, Western Blot, Expressing, Activity Assay, Staining

Journal: International Journal of Molecular Sciences

Article Title: Rice Peroxygenase-9 Negatively Regulates Production of Reactive Oxygen Species and Increases Cellular Resistance to Abiotic Stress

doi: 10.3390/ijms26146918

Figure Lengend Snippet: Accumulation of ROS and cell death level in rice seedlings from WT and ox lines under drought and salt stress. ( A ) DAB staining, ( B ) NBT staining, and ( C ) Evan’s blue staining. Three-week-old seedlings grown in soil were subjected to stressors, as follows: For drought stress, seedlings were taken out and dried in the air at room temperature for 4 h (DAB and NBT staining) or 6 h (Evan’s blue staining); for salt stress, 200 mM NaCl was added directly to the water reservoir containing the rice seedling pots for 24 h (DAB and NBT staining) or 36 h (Evan’s blue staining). All the treated rice seedlings were re-subjected to the normal water condition for 12 h to recover before staining with 0.2% DAB ( w/v ) solution (24 h), 0.1% ( w/v ) NBT solution (24 h), or 0.25% Evan’s blue ( w/v ) solution (30 min), followed by boiling in 95% ethanol for 4 h and observed under a light microscope with 10× magnification. Non-treated seedlings were used as the control. White bar = 1 cm and black bar = 1 mm. The stained area was converted from three separated microscopic photos using ImageJ Version 1.45p. ( D ) Malondialdehyde (MDA) content in the leaves of WT, ox2-1, and ox2-2 under drought and salt stress. Error bars denote ± SD of three replicates. The p -values were calculated using the Student’s t -test (** p < 0.05 and *** p < 0.01).

Article Snippet: For MDA content quantification, third leaves of the WT and stress-treated seedlings were collected, and MDA content was extracted and measured by the Malondialdehyde (MDA) Colorimetric Assay Kit (Cat. #E-BC-K025-S, Elabscience, Houston, TX, USA).

Techniques: Staining, Light Microscopy, Control